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I *Finally* had my committee reunion.
I'm an absolute failure, you know?
It's only that I cannot think or imagine how controls will make my life easier.
It results that I have to prove that both my genes respond to viral infection by amplifying each one of the viral genes... A little bit over the top for me, but they are the law..
and well, it results also that either one of my mutants has a functional copy of the gene or the mutation, even if homozygous, is not affecting the overall phenotype because there is another rescuing gene or the insertion is way before the transcription initiation site.
so I have to verify if the mutant *is* a mutant, or a useful one, at least.
:S
The good news are that I no longer have to produce my own proteins in order to make antibodies. My boss went generous this semester and decided that everything will be synthesized by an independent lab, that means we'll order them! Yay!
So a semester full of Real-time RT-PCRs, synthetic peptides, mutants and infection kinetics is waiting for me.
well, that and a raging boss in his office. I must go or....
(*marcha goes expecting not to be yelled at ... well.. not that much)
See ya all!
P.d. I love my nerdicon! XDDDDDDDDDDDDDDD
I'm an absolute failure, you know?
It's only that I cannot think or imagine how controls will make my life easier.
It results that I have to prove that both my genes respond to viral infection by amplifying each one of the viral genes... A little bit over the top for me, but they are the law..
and well, it results also that either one of my mutants has a functional copy of the gene or the mutation, even if homozygous, is not affecting the overall phenotype because there is another rescuing gene or the insertion is way before the transcription initiation site.
so I have to verify if the mutant *is* a mutant, or a useful one, at least.
:S
The good news are that I no longer have to produce my own proteins in order to make antibodies. My boss went generous this semester and decided that everything will be synthesized by an independent lab, that means we'll order them! Yay!
So a semester full of Real-time RT-PCRs, synthetic peptides, mutants and infection kinetics is waiting for me.
well, that and a raging boss in his office. I must go or....
(*marcha goes expecting not to be yelled at ... well.. not that much)
See ya all!
P.d. I love my nerdicon! XDDDDDDDDDDDDDDD
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