Marchatorium

After an uneventful weekend, today was a very busy day. I bombarded a tray of plants, to assess if infection is developed faster in a certain mutant I'm working on. :D
Then, I had to empty 6 trays of old, dessicated  plants that I was postponing, and were abandoned in a corner of my growth chamber. It was a messy job. Then I went to the greenhouse, asked for two bags of fresh Arabidopsis mix and prepared it, and then refilled the previously cleaned trays. :S Tomorrow I'll be sowing the little brats from solid Agar MS medium to soil, and preparing the bombardment of two more trays. :D I expect this to be one of the final steps of experimentation.

Went to see The Bourne Ultimatum yesterday. Surprisingly, it pleased me. I'm not a Damon fan, he seems a little slow to me, but I actually liked the movie and his acting as the hero.

Last week saw Knocked Up. Soooo funny! And the main character is so cute! Seth Rogen is his name, I think... well, a bit in the "dirty" side, but still... I liked him. *winks* So I bought The 40 Year Virgin, just for seeing the man again xD.

It's raining heavily right now... and it has been like that all this week. I have to do my laundry, and the indoor clotheslines look awful. Suggestions? (I don't have a dryer :P)


Ah, and now a  song that fits painfully well to my current situation. *headdesks*



I just hate men. 

First, Sorry about not commenting and everything. I've had lots of work...
First, I had to plan a Southern Blot experiment I'm planning to do next week, implying bombarding Wild type and Mutant plants, extracting their DNA this time, blotting it into a membrane, and hybridize that membrane against viral genomes.
Then , to perform the Real time PCR experiments I have been dragging for a semester.
That means lots of efforts because I have to make sure that my controls amplify correctly, that the plasticware and pipettes are completely clean, that my synthesized cDNA is fresh and suitable, etc etc etc.... I'm sick of it.

Other news: The dog's name is Sauron (officially) but everyone names and calls it whatever they want so ... I quit. Name my pet as you wish.

My social life has been as deserted as the sahara. I don't know why but no parties, no bar outings, nothing this month! I NEED PARTY!! I SERIOUSLY need it. I hate to drink alone, and I need a drink.  I want to dance, to flirt, everything that makes a party a real fun one.

Lenin: Ah, a bit better, he's still abroad but he loves me, or at least he says so. I'm trying to keep the love in myself.

Ah, and the sims 2 were back in my life. I'm currently playing myself married to Carlos D. Oh yeah, he's a sim cutie, as his real self. and I'm just gorgeous, the problem is that when his custom made skin combines to make children, the children look simply odd. :P so no cute kids for us.
I'll try to have a new sim family with me and joaquin phoenix. ^^ I expect a better outcome for our kids then xD

Love to everyone.

for those new, I HATE WARM SEASON! I cannot stand temperatures above 32ºC or so... and thus, I decided to work the night shift. It's a bit risky because in our lab is strictly forbidden to work alone, for safety reasons, but I seriously  melt in my bench, so I have to work when sun is out and everywhere is quiet and about at 25ºC ^_______^
Now, 4:13 a.m. I'm finishing RNA extraction from 60 samples, I expect to have everything ready for RT-PCR approximately 10 a.m....Gel electrophoresis included. Yay me!
Btw: I have to attend a wedding next saturday and I have nothing to wear... I'm  doubting if I'll assist or not.  *yawns*

^^
Love the nights....
love everyone
Have a nice weekend! ^______^

Yes. I'm weak. I went to the party, alright? I have no word, period. :S
Fuck!
I DIDN'T drink anything, though. Well, a couple of glasses of wine but that was it. Non-drunk, healthy dancing. What it bothered me a little was the fact that the only music played was "grupera" (ok, let me explain that. tropical music, or "banda, norteña, reggaeton" music. If you don't know what it is, do not experiment. it's very good for dancing, but boring as hell) so I got tired very soon... and left. Alone. I miss Lenin in such walking in the dark street cases.
I should cut on the smoking pace. I had almost two packs today.  I love my cigarettes. I've lived with them since I was a toddler... how possibly hate them?
tomorrow: My first Gus-staining results. I'm nervous.
blah.
Good night, people. try to sleep... I'll do. =D

I bombarded 350 of my Arabidopsis babies today, three lines, 115 plants per line. That meant 2 hours of preparation (covering gold particles with the different constructs,  tags, transport of the plant trays, etc) and 4 additional shooting hours. I'm wrecked. Samplings will begin tomorrow and they'll finish on Sunday morning, then I'm departing to see milosflaca and lummie, yipee!
When I return I expect symptoms from every virus-shot plant. :)

Other stuff:
Lenin called me yesterday  from a party, and he was drunk, again (tell me something new :S) . I'm fearing he's drinking a bit too much. (5 days a week that's what I call an excess) He told me that in France is very common  and that they are allowed to drink everywhere, even in the laboratories, and I'm reluctant to believe so. For me, that's nostalgia mixed with beer and self-abandon and lab stress. And, again, the old story about sharing his life with me and blah blah blah... I hate that he never tells me he can't live without me when sober. Or maybe he tells me but a lot less adamant and vehement.

and... I discovered a bunch of new freckles on my skin. I'm depressed. I hate sun.

Ok, hugs to everyone. Or kisses. choose yourself, I'm generous xD. :P

My little babies are not ready to be inoculated...
That adds a whole week to my schedule.  They have only four true leaves, and I have to wait until they have at least 6, because if not, the infection can delay even more than 15 days... (that because the god damn virus needs short days (8hlight-16h darkness) and a STABLE 23ºC temperature and blah blah blah)
What calms me is that I have everything (Viral clones and gold particles and growth chamber) ready and just when they reach the appropriate size and developmental stage, I'll be bombarding them right away, because I NEED THOSE RESULTS NOW!  and  the infection is only the first step, I need to take samples of all three lines to make Real-time PCR assays, Gus stainings and complementations...
Jesus!
And the news are: The NPK1-Salk is not really a mutant line, as the insertional homozygous are lethal, I cannot segregate a truly mutant line.
So I have to order a new line from ABRC. :S NPK1 is giving me headaches, because the promoter:reporter gus expressing lines do not express said gene further  than in T1 population, as the gus gene and Km gene seem to be silenced,  ergo, I'm transforming AGAIN (Three times with this) to have seedlings to work with. I HATE FLORAL DIP! ¬____¬ is so messy... but I prefer that than vacuuming or tissue-culture.
anyway I'm sorry about that lab rant, If you got dizzy for reading this, my apologies.

And.... a music recommendation: Kaiser Chiefs. I'm IN ENDLESS LOVE with them. (Lovely British accents, superb music, emo, despaired lyrics...*faints again*)


Edit: hooooo!! No lab meeting today!! Yahoo!!!

Well, I was looking for some nice Christopher Meloni and /or Carlos Dengler  (or Carlos D, whatever the prince wants to be called)  pics, hi-res... for desktop crafting.. _(I'm sorta tired of Joaquin Phoenix... unbelievable, uh?)  Does anyone has some? or one? or lots? If so, I'd be so thankful!
I swear I spent a whole hour surfing the net but my results (obviously due my lack of searching abilities and method) were null.
Or if anyone knows how to search in places like photobucket or imageshack for specific images? anyway I'd be so so so in debt I promise I'll cook or do something for whoever helps me solve my prob xD

Other stuff:

And my boss is still absent! (I hope he forgets yesterday meeting and the subsequent rage hehehe)
hugs for everybody!

Yes, I know. I'm such a whiner. I have to share this with anyone, I guess. Everyone in the lab has several responsibilities. Preparing a number of common solutions, sterilize glassware, making sure there are enough culture media, etc. Mine is to prepare all the antibiotics and other additives for culture media, such as Kanamycin, Carbenicillin, Nalidixic Acid, Tetracycline, IPTG, X-Gal, X-Glu, etc etc, and to make sure there is plenty of them ready to use.
To prepare carbenicillin is a very simple task. Glass vials with premeasured quantities of cb are injected with 10ml sterile double-distilled deionized water, the resultant mix aliquoted into 1 ml eppendorf tubes, and frozen.
But today instead of injecting the water to the vial, I pricked myself with the needle, down to the bone, I reached it I swear, and since the needle is rather thick, now I have a hole in my left index finger, and it hurts!! T____T Yes I know I'm such a sissy.

I want my mommy! I want Lenin! I just want a hug. TOT